insight into the molecular mechanisms by which Sum1p, a transcriptional
repressor of S. cerevisiae, suppresses defects in α-tubulin folding.
Whole genome transcript profiling of tubulin lacking and tubulin
overexpressing yeast cells identified Sum1p as a stress response
regulator in yeast. No tubulin or microtubule-specific gene was found
to be differentially regulated.
Further questions: how does SUM1-mediated dosage suppression of tub-1
1. Metabolic regulation? Does down
regulation of transcription and
specifically of several GTPases lead to increased intracellular levels
of GTP known to be critical for α-tubulin and β-tubulin folding?
2. How does the
transcriptome differ in the absence of Hst1 and Sir2
3. How does
acetylation affect the proteome?