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Transcriptomics ________________________________________________


Pratima Sinha


Understand transcriptional networks controlling microtubule stability in the budding yeast


To gain insight into the molecular mechanisms by which Sum1p, a transcriptional repressor of S. cerevisiae, suppresses defects in α-tubulin folding .

Whole genome transcript profiling of tubulin lacking and tubulin overexpressing yeast cells identified Sum1p as a stress response regulator in yeast. No tubulin or microtubule-specific gene was found to be differentially regulated.

Further questions: how does SUM1-mediated dosage suppression of tub-1 mutations occur?

1. Metabolic regulation? Does down regulation of transcription and specifically of several GTPases lead to increased intracellular levels of GTP known to be critical for α-tubulin and β-tubulin folding?


2. How does the transcriptome differ in the absence of Hst1 and Sir2 proteins?


3. How does acetylation affect the proteome?



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